Gene expression from RNA-seq of different ESR1 mutation cell models

RNA-seq data were extracted from three resources: Steffi Oesterreich (SO) Bahreini et al. Breast Cancer Research, 2017 #GSE89888 Y537S/D538G mutations were knocked in MCF7 and T47D cells with rAAV and CRISPR/Cas9 respectively. Genome-edited cells were hormone deprived and treated with or without 1 nM E2 for 24 hours. RNA were extraced and processed for RNA-seq. Simak Ali (SA) Harrod et al. Oncogene, 2017 #GSE78286 Y537S mutation was knocked in MCF7 cells with CRISPR/Cas9. Genome-edited cells were hormone deprived and treated with or without 1 nM E2 for 8 hours. RNA were extraced and processed for RNA-seq. Myles Brown (MB) Jeselsohn et al. Caner Cell, 2018 #GSE94493 Dox-inducible Y537S or D538G plasmids were stably expressed in MCF7, Y537S mutant was expressed in T47D. Cells were hormone deprived and treated with or without 1 nM E2 for 24 hours. RNA were extraced and processed for RNA-seq.